Theknoloji ea Moloko o Latelang e Potlakisa Tlhahiso ea Mohlala oa Mouse
Theknoloji ea TurboMice™
E ntlafalitsoe ke MingCeler ka letoto la lintlafatso tsa tlatsetso ea tetraploid.
Ka ho kopanyaTheknoloji ea Precise Gene Editingle toeba e ntlafalitsoengTheknoloji ea ho lokisetsa Embryonic Stem Cell, joale re ka hlophisa hoo e batlang e le sebaka sefe kapa sefe sa lefutso.
Theknoloji ea TurboMice™
Theknoloji ea rona e sebetsang hantle haholo ea Tetraploid Complementation e re lumelletse ho eketsa haholo sekhahla sa tsoalo ea litoeba ho tloha ho 1% -5% ho ea ho 30% -60% ka lisele tsa stem tse hlophisitsoeng ka liphatsa tsa lefutso, tse batlang li lekana le katleho ea phetisetso e tloaelehileng ea embryo.
MingCeler ke k'hamphani ea pele lefats'eng e atlehileng ho fihlela phetoho ea theknoloji ea Tlatsetso ea Tetraploid ho tloha laboratoring ho ea ho ts'ebeliso ea indasteri.
Theknoloji ea Setso
Theknoloji ea setso ea mohlala oa liphoofolo, joalo ka pronuclear microinjection le ES targeting, e kenyelletsa ho tsoalisa bonyane meloko e 2 ho isa ho 3 ho fumana litoeba tsa mofuta oa homozygous, tseo hangata li nkang likhoeli tse 6-8.
Ts'ebetso ena e telele e sitisa haholo tsoelopele le katleho ea nts'etsopele ea lithethefatsi tse ncha le lipatlisiso tsa mafu.
Sehlopha se Potlakileng
1. ho fihla ho litoeba tse 20 tse hlophisitsoeng ka liphatsa tsa lefutso tsa homozygous ka hareLikhoeli tse 2-4.
2. ≈ 50 litoeba homozygous ka hare ho tse latelangLikhoeli tse 2.
3. 400+ litoeba tsa homozygous ka hare8-12 likhoeli.
Tlaleho ea nyeoe ka 2020
Theknoloji ea TurboMice ™ e ile ea sebelisoa ho hlahisa ka potlako500 homozygouslitoeba tsa ACE2 tse entsoeng ke batho ka har'a feelaLikhoeli tse 8bakeng sa lithuto tsa lithethefatsi le tsa ente ea COVID-19.
Flexible Strain Selection
Mekhoa ea setso e na le mefokolo e kholo khethong ea mofuta oa litoeba, athe theknoloji ea TurboMice™ e fana ka maemo a feto-fetohang ka mefuta e fapaneng ea mefuta e fapaneng eo u ka khethang ho eona (ho kenyeletsoa Balb / c, ICR, C57BL/6, joalo-joalo).
● Litoeba tsa mofuta oa F0 tse entsoeng ka theknoloji ea TurboMice™ ke litoeba tse hlasetsoeng tse nang le sele e le 'ngoe.
● Ka hona, liphatsa tsa lefutso tsa litoeba tsa F0 li tšoana, ho fokotsa liphoso tsa liteko tse bakoang ke ho fapana ha liphatsa tsa lefutso.
● Ka hona, lintlha tsa liteko bakeng sa tlhahlobo ea katleho ea lithethefatsi le liteko tse ling lia lumellana ebile lia tšepahala.
E Boloka Botšepehi bo Molemo ba Liphatsa tsa lefutso
Ho Situ Precision Gene Editing
● Ho fetola liphatsa tsa lefutso ka mokhoa o nepahetseng o nang le maemo a nepahetseng a ho hlahisa liphatsa tsa lefutso le lisele tse nepahetseng.
● Litoeba tsa ACE2 tse entsoeng ke batho ka mekhoa ea khale li hlahisoa ke kenyelletso ea khale ea mothetsi oa K18-ACE2, e sa khoneng ho etsa liphetoho tse nepahetseng ka lebaka la ho kenyeletsoa ka tšohanyetso, ho bakang khaello ea sebopeho sa ACE2 mofuteng o entsoeng ke motho oa ACE2. .
● Litoeba tsa ACE2 tsa MingCeler li bonts'a ponahalo e ikhethang likarolong tse fapaneng tsa 'mele (litšoantšo tsa C le D ka holimo), 'me li etsisa litšobotsi tsa bophelo bo botle ka mor'a tšoaetso ea SARS-CoV-2.
Sethala se ikhethileng sa EnhancerPlus
●Sethala sa thepa sa MingCeler sa EnhancerPlus se ka thusa bareki ba rona ho ntlafatsa maemo a polelo a mofuta oa motho.
● Ka lebaka la lilemo tse ngata tsa ho bokella boitsebiso ho epigenetics le lipatlisiso tsa qeto ea hore na ho etsahala'ng ka lisele, sethala sa MingCeler's EnhancerPlus se ka bolela esale pele ka nepo maemo a genomic a Enhancers, a etsa hore moralo oa maano a ho fetola liphatsa tsa lefutso a thehoe bopaking.
● Ka lebaka leo, liphatsa tsa lefutso tseo ho lebelloang ho tsona li ka tšoana hantle le mekhoa ea ho hlahisa maikutlo le maemo a liphatsa tsa lefutso tse sa feleng.
Tlaleho ea nyeoe: Humanization ea X gene.
● Ho sebelisa sethaleng sa EnhancerPlus, leano le ile la ntlafatsoa 'me boemo ba polelo bo ile ba bona keketseho ea litaelo tse tharo tsa boholo (sheba setšoantšo se ka holimo), ho fihlela litlhoko tsa nts'etsopele ea lithethefatsi joalokaha ho khethiloe ke moreki boemong ba protheine.
● Kamora ho phethela tlhahiso e kholo ea lefats'e ea litoeba tsa ACE2 ka 2020, MingCeler e ntlafalitse mofuta oa toeba oa ACE2 ka makhetlo a mane ka EnhancerPlus optimization, 'me boemo ba polelo ea ACE2 e entsoeng ke motho e ile ea qetella e fihlile haufi le polelo ea khale ea toeba ACE2.
Maemo a polelo a ACE2 e entsoeng ke motho likarolong tse fapaneng tsa mefuta ea litoeba tsa motho
Multi-Locus Gene Editing
● Theknoloji ea TurboMice™ ke khetho e ntle ka ho fetisisa 'marakeng bakeng sa ho hlahisa mefuta e mengata ea litoeba tsa combinatorial gene-edited gene-edited, e fanang ka melemo ea ho nepahala ho phahameng ho kenya sebaka, ho kenya likaroloana tse telele, le libaka tse ngata tse fetotsoeng ka liphatsa tsa lefutso, ntle le mathata a ho arohana.
● Theknoloji ea TurboMice™ e lumella ho hlophisa ka nako e le 'ngoe ho fihla ho liphatsa tsa lefutso tse 3 ho hlahisa mefuta e mengata ea li-homozygous multi-locus gene-edited mouse ka ho toba ho tloha liseleng tsa stem tse hlophisitsoeng ka liphatsa tsa lefutso, ntle le tlhokahalo ea mekhoa e senyang nako ea ho ikatisa, ho fana ka mefuta e fapaneng. ea mefuta e rarahaneng ea boleng bo holimo e hlokahalang bakeng sa lipatlisiso tse ncha tsa lithethefatsi.
● MingCeler e atlehile ho hlahisa mefuta e fapaneng ea litoeba tse fetotsoeng liphatsa tsa lefutso tse ngata ka nako e khuts'oane, ho ipapisitsoe le litoeba tsa ACE2 tse entsoeng ke batho.
Ho fetola liphatsa tsa lefutso tse telele
Theknoloji ea TurboMice™ e thusa ho hlophisa ka nepo liphatsa tsa lefutso tsa likarolo tse telele tse fetang 20kb, ho thusa tlhahiso e potlakileng ea mefuta e rarahaneng joalo ka humanization, conditional knock-out (CKO), le big fragment knock-in (KI).
Lingoliloeng
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin e tlama ACE2 ho thibela tšebelisano ea eona le SARS-CoV-2 spike protein mme e sebetsa hantle ho thibela tšoaetso ea SARS-CoV-2 mefuteng ea liphoofolo.Lisele Res.2021 Jan;31(1):17-24.doi: 10.1038/s41422-020-00450-0.( HAEBA: 20.507 )
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT, Chen J. Moloko o potlakileng oa ACE2 oa mofuta oa toeba oa motho bakeng sa COVID-19 o tlatselletsang tetraploid.Natl Sci Rev. 2020 Nov 24;8(2):nwaa285.doi: 10.1093/nsr/nwaa285.( HAEBA: 16.693 )
Phallo ea Tšebeletso
Seo u hlokang ho se etsa feela ke ho re fa tlhoko ea ho fetola mofuta oa mofuta oa mouse ea hau.Ho ipapisitsoe le litlhaloso tsa hau, re tla kopanya moralo o ikhethileng oa selelekela, 'me ka mor'a lipuisano tse ling le tumellano ea bobeli, re tla saena konteraka ea tšebeletso ea tekheniki.Ka mor'a hore morero o qale, re tla fana ka lintlafatso tse nakong mabapi le tsoelo-pele.