QuickMice™ ke eng?

Ho boleloa hore sele e homozygous bakeng sa lefutso le itseng ha li-allele tse tšoanang tsa lefutso li le teng ho li-chromosome tsa homologous.

Mefuta ea litoeba tse entsoeng ke batho e na le mefuta e mengata ea ts'ebeliso mafapheng a lipatlisiso a AIDS, mofets'e, mafu a tšoaetsanoang le lefu la mali.

Knock-in (KI) ke mokhoa o sebelisang motsoako oa homologous oa liphatsa tsa lefutso ho fetisetsa mofuta o sa tloaelehang oa lefutso ka tatellano ea homologous ka sele le genome, le ho fumana polelo hantle ka seleng ka mor'a ho kopana hape.

Conditional Knock-out (CKO) ke tekhenoloji e ikhethileng ea lefutso e fumanehang ka mokhoa oa ho kopanya bocha.

Ka ho etsa kopoTurboMice™theknoloji, re ka khona ho hlahloba ka ho toba lisele tsa embryonic stem ka mor'a ho fetola liphatsa tsa lefutso ka matsatsi a 3-5, ebe re haha ​​​​sele ea tetraploid, 'me ra fumana litoeba tse hlophisitsoeng ka liphatsa tsa lefutso tse ngata ka likhoeli tse 3-5 ka mor'a ho beleha ke litoeba tsa' mè, tse ka bolokang selemo se le seng. bakeng sa bareki ba rona.

TurboMice™thekenoloji e thusa ho hlophisa ka nepo liphatsa tsa lefutso tsa likhechana tse telele ho feta 20kb, ka hona ho thusa tlhahiso e potlakileng ea mefuta e rarahaneng joalo ka humanization, conditional knockout (CKO), le kholo ea fragment knock-in (KI).