Lisele tse hlophisitsoeng ka liphatsa tsa lefutso
Lisele tse fetotsoeng ka liphatsa tsa lefutso li bua ka lisele tse bileng le liphetoho tsa liphatsa tsa lefutso ka mokhoa o tsejoang e le phetoho ea liphatsa tsa lefutso.Ho fetola liphatsa tsa lefutso ho kenyelletsa ho etsa liphetoho ho DNA ea ntho e phelang, hangata ka sepheo sa ho ithuta ka liphatsa tsa lefutso tse itseng kapa ho utloisisa mekhoa e itseng ea tlhaho.
U sebelisa theknoloji ea ho fetola liphatsa tsa lefutso, hoa khoneha ho finyella mela ea lisele tsa liphatsa tsa lefutso, lisele tse kokotang, phetoho ea lintlha, kapa lisele tsa lisele tsa liphatsa tsa lefutso ka liseleng tse itseng, tse ka sebelisoang ho ithuta liphatsa tsa lefutso, lipontšo, mekhoa ea mafu. , le ntlafatso ea lithethefatsi, hammoho le ho tšoaea lisele tse itseng.
Ho na le mabaka a 'maloa a etsang hore mela e hlophisitsoeng ea liphatsa tsa lefutso e be ea bohlokoa lipatlisisong tsa mahlale.Taba ea pele, ba fana ka sesebelisoa sa ho ithuta tšebetso ea liphatsa tsa lefutso tse itseng.Ka ho fetola liphatsa tsa lefutso sebakeng se laoloang, bo-rasaense ba ka bona phello ea liphetoho tsa liphatsa tsa lefutso boitšoarong ba lisele kapa mekhoa e meng ea baeloji.Tsebo ena e ka kenya letsoho kutloisisong e molemo ea mafu a liphatsa tsa lefutso, hammoho le ho tseba lipheo tse ka 'nang tsa e-ba teng tsa phekolo.
Ho feta moo, lisele tse hlophisitsoeng ka liphatsa tsa lefutso li ka sebelisoa ho hlahisa le ho hlahloba lithethefatsi tse ncha.Ka ho hlahisa liphetoho tse khethehileng tsa liphatsa tsa lefutso, bo-ramahlale ba ka etsa mela ea lisele e etsisang maemo a mafu, e ba nolofalletsang ho lekola katleho ea liphekolo tse ka bang teng.Sena se lumella hore ho be le mekhoa e nepahetseng le e sebetsang ea ntlafatso ea lithethefatsi.
Ho feta moo, mela e hlophisitsoeng ea liphatsa tsa lefutso e na le monyetla oa ho sebelisoa meriana ea tsosoloso.Ka ho fetola liphatsa tsa lefutso tse ikarabellang bakeng sa boitšoaro ba lisele kapa karohano, bo-ramahlale ba ka ntlafatsa matla a kalafo a lisele tsa stem kapa ba etsa lisele tse loketseng ho fetisoa.
Molemo
1. Li-locus tse ngata tse hlophisitsoeng ka liphatsa tsa lefutso ka nako e le 'ngoe;
2. ho fana ka ts'ebeletso e le 'ngoe ho tloha ho moralo oa sgRNA le ho kopanya ho ea ho tlhahlobo ea lisele;
3. Boiphihlelo bo ruileng ba ho kopanya liphatsa tsa lefutso ho thusa liphatlalatso tsa ho fetola liphatsa tsa lefutso ho tloha ho moralo oa morero ho fihlela ho phethoa ha mohaho.
Khoebo e ka Sehloohong
- Gene Knock-out Cell Lines
- Gene Activation Cell Lines
- Point mutant cell line
- Knock-in Cell Lines
Litšupiso
[1] Zhang S, Shen J, Li D, Cheng Y. Mekhoa ea ho fana ka Cas9ribonucleoprotein bakeng sa ho hlophisoa ha genome ea CRISPR/Cas9.Theranostics.2021 Jan1;11(2):614-648.doi: 10.7150/thno.47007.PMID: 33391496;PMCID: PMC7738854.
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